RESUMO
The genus Vibrio includes pathogenic bacteria able to cause disease in humans and aquatic organisms, leading to disease outbreaks and significant economic losses in the fishery industry. Despite much work on Vibrio in several marine organisms, no specific studies have been conducted on Anadara tuberculosa. This is a commercially important bivalve species, known as "piangua hembra," along Colombia's Pacific coast. Therefore, this study aimed to identify and characterize the genomes of Vibrio isolates obtained from A. tuberculosa. Bacterial isolates were obtained from 14 A. tuberculosa specimens collected from two locations along the Colombian Pacific coast, of which 17 strains were identified as Vibrio: V. parahaemolyticus (n = 12), V. alginolyticus (n = 3), V. fluvialis (n = 1), and V. natriegens (n = 1). Whole genome sequence of these isolates was done using Oxford Nanopore Technologies (ONT). The analysis revealed the presence of genes conferring resistance to ß-lactams, tetracyclines, chloramphenicol, and macrolides, indicating potential resistance to these antimicrobial agents. Genes associated with virulence were also found, suggesting the potential pathogenicity of these Vibrio isolates, as well as genes for Type III Secretion Systems (T3SS) and Type VI Secretion Systems (T6SS), which play crucial roles in delivering virulence factors and in interbacterial competition. This study represents the first genomic analysis of bacteria within A. tuberculosa, shedding light on Vibrio genetic factors and contributing to a comprehensive understanding of the pathogenic potential of these Vibrio isolates.IMPORTANCEThis study presents the first comprehensive report on the whole genome analysis of Vibrio isolates obtained from Anadara tuberculosa, a bivalve species of great significance for social and economic matters on the Pacific coast of Colombia. Research findings have significant implications for the field, as they provide crucial information on the genetic factors and possible pathogenicity of Vibrio isolates associated with A. tuberculosa. The identification of antimicrobial resistance genes and virulence factors within these isolates emphasizes the potential risks they pose to both human and animal health. Furthermore, the presence of genes associated with Type III and Type VI Secretion Systems suggests their critical role in virulence and interbacterial competition. Understanding the genetic factors that contribute to Vibrio bacterial virulence and survival strategies within their ecological niche is of utmost importance for the effective prevention and management of diseases in aquaculture practices.
Assuntos
Arcidae , Sistemas de Secreção Tipo VI , Vibrio parahaemolyticus , Animais , Humanos , Virulência/genética , Fatores de Virulência/genética , AntibacterianosRESUMO
Conventional techniques described in the literature for the manufacture of ocular prostheses are time-consuming since they involve manual work. The use of technologies could improve this laborious process, providing better esthetic outcomes. This technique describes how to manufacture the ocular portion of an orbital prosthesis using a smartphone camera, color calibration with a tooth shade guide, and digital printing. This method allows clinicians to fabricate customized ocular prosthesis by using a photograph of the patient's eye, thus eliminating the need for hand-painting and manual work, and reducing fabrication time.
Assuntos
Estética Dentária , Smartphone , Calibragem , Cor , Humanos , Impressão Tridimensional , Desenho de PróteseRESUMO
In mesenchymal stem cells (MSCs), it has been reported that prostaglandin E2 (PGE2) stimulation of EP2 and EP4 receptors triggers processes such as migration, self-renewal, survival, and proliferation, and their activation is involved in homing. The aim of this work was to establish a genetically modified adipose (aMSC) model in which receptor genes EP2 and EP4 were edited separately using the CRISPR/Cas9 system. After edition, the genes were evaluated as to if the expression of MSC surface markers was affected, as well as the migration capacity in vitro of the generated cells. Adipose MSCs were obtained from Chilean breed horses and cultured in DMEM High Glucose with 10% fetal bovine serum (FBS). sgRNA were cloned into a linearized LentiCRISPRv2GFP vector and transfected into HEK293FT cells for producing viral particles that were used to transduce aMSCs. GFP-expressing cells were separated by sorting to obtain individual clones. Genomic DNA was amplified, and the site-directed mutation frequency was assessed by T7E1, followed by Sanger sequencing. We selected 11 clones of EP2 and 10 clones of EP4, and by Sanger sequencing we confirmed 1 clone knock-out to aMSC/EP2 and one heterozygous mutant clone of aMSC/EP4. Both edited cells had decreased expression of EP2 and EP4 receptors when compared to the wild type, and the edition of EP2 and EP4 did not affect the expression of MSC surface markers, showing the same pattern in filling the scratch. We can conclude that the edition of these receptors in aMSCs does not affect their surface marker phenotype and migration ability when compared to wild-type cells.
RESUMO
PURPOSE: To measure dimensional changes of the periimplant soft tissue profile after removal of a single implant fixed interim restorations using digital impression procedures. MATERIALS AND METHODS: Ten participants presenting with single implant-supported fixed interim restorations (ISFIRs) on the maxillary esthetic zone. A 2-step silicone impression was made of the maxillary arch with the ISFIRs. The experimental procedure was obtained by making digital impressions of the gingival contours immediately after ISFIR removal. The control procedure was formed by fabricating definitive casts from the conventional impression using the ISFIRs as a customized impression transfer and making digital impressions of these definitive casts. Both images of paired groups were digitally overlapped on the computer, and their profiles were measured at the coronal, midlevel gingiva in the buccolingual and mesiodistal width. RESULTS: Statistically significant differences between the ISFIR emergence profile width and the unsupported soft tissue profile width were observed at the midlevel gingiva in the buccolingual dimension (1.35 mm) and at the coronal (0.51 mm) and midlevel gingiva (1.29 mm) in the mesiodistal dimension. CONCLUSIONS: A digital impression, as used in this pilot study, does not capture accurately the desired soft tissue dimensions immediately after removal of the ISFIR.
Assuntos
Prótese Dentária Fixada por Implante , Restauração Dentária Temporária , Gengiva/patologia , Adulto , Idoso , Técnica de Moldagem Odontológica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Projetos PilotoRESUMO
In recent decades there has been an important academic debate on how to transfer research-based knowledge to the health policy-making process. Despite an intensive controversy and various approaches to the problem, there is basic consensus concerning both the academic relevance and the usefulness of further evaluation of concrete experiences that allow conclusions and clarification of what are still confusing issues. The current article discusses a joint research experience by professors and local and regional health policy-makers. The research focused on the socioeconomic conditions, duration of the employment/unemployment cycle, and features of health insurance for workers that lost their jobs in the Greater Metropolitan "Valle de Aburrá" Area (Antioquia, Colombia) in 2005, as the basis for a proposal to guarantee the maintenance of health insurance for these workers. The potentialities and difficulties of the interactive process are analyzed. The article is also intended to elucidate how scientific outcomes influence policymaking in health, as well as the difficulties imposed by concrete reality and the political process for implementing the innovative proposal.
Assuntos
Pesquisa Biomédica , Política de Saúde , Seguro Saúde , Saúde Ocupacional , Formulação de Políticas , Desemprego , Pessoal Administrativo , Colômbia , Difusão de Inovações , Reforma dos Serviços de Saúde , Humanos , Relações Interprofissionais , Pesquisadores , Fatores SocioeconômicosRESUMO
A pesar del debate académico acerca de la utilización de los resultados de las investigaciones en el proceso de formación de políticas públicas en salud, existe consenso sobre la relevancia del tema y la necesidad de analizar experiencias que ayuden a clarificar aspectos todavía confusos. Este artículo presenta la experiencia de trabajo conjunto entre investigadores académicos y tomadores de decisión políticos en salud del nivel regional y local. El estudio estableció las condiciones socioeconómicas, el ciclo empleo-desempleo y las características del aseguramiento en salud de los trabajadores cesantes en el Area Metropolitana del Valle de Aburrá (Antioquia, Colombia) en el año 2005. Con base en los resultados se elaboró una propuesta de intervención que busca garantizar la sostenibilidad de su aseguramiento en salud y de sus familias. Se analizan las potencialidades y dificultades del proceso de interacción y se discute la influencia que pueden tener los resultados de la investigación en la formación de políticas públicas en salud, como también las dificultades que impone la realidad concreta y el proceso político para la implementación de la innovación propuesta.
In recent decades there has been an important academic debate on how to transfer research-based knowledge to the health policy-making process. Despite an intensive controversy and various approaches to the problem, there is basic consensus concerning both the academic relevance and the usefulness of further evaluation of concrete experiences that allow conclusions and clarification of what are still confusing issues. The current article discusses a joint research experience by professors and local and regional health policy-makers. The research focused on the socioeconomic conditions, duration of the employment/unemployment cycle, and features of health insurance for workers that lost their jobs in the Greater Metropolitan "Valle de Aburrá" Area (Antioquia, Colombia) in 2005, as the basis for a proposal to guarantee the maintenance of health insurance for these workers. The potentialities and difficulties of the interactive process are analyzed. The article is also intended to elucidate how scientific outcomes influence policymaking in health, as well as the difficulties imposed by concrete reality and the political process for implementing the innovative proposal.
